Next Generation Sequencing
We offer next generation sequencing services on our Illumina NovaSeq 6000 and Illumina MiSeq. The combination of these systems provides us with a wide degree of flexibility in terms of read length and depth, allowing us to quickly sequence small amplicon-based sequencing projects as well as larger whole-genome scale projects.
Overview of Illumina Sequencing by Synthesis Workflow
NovaSeq 6000
The NovaSeq combines throughput and flexibility for virtually any method, genome, and scale; supporting diverse applications such as large whole-genome sequencing, exome and large panel sequencing, single-cell profiling, transcriptome sequencing, and more. It utilizes a variety of flow cell types (SP, S1, S2, S4) to provide the broadest output range. The NovaSeq 6000 System generates from 80Gb/800M reads to 3Tb/10B reads of data in single flow cell mode. In dual flow cell mode, output can be up to 6Tb and 20B reads.
| Flow Cell Type | SP | S1 | S2 | S4 |
| Lanes per Flow Cell | 2 | 2 | 2 | 4 |
| Clusters PF | 0.65 – 0.8 billion | 1.3 – 1.6 billion | 3.3 – 4.1 billion | 8 – 10 billion |
| Available Run Configurations | ||||
| PE50, SR100 (100 cycle) | Yes | Yes | Yes | – |
| PE100 (200 cycle) | Yes | Yes | Yes | Yes |
| PE150 (300 cycle) | Yes | Yes | Yes | Yes |
| PE250 (500 cycle) | Yes | – | – | – |
MiSeq
The MiSeq is a smaller unit used primarily for small whole-genome sequencing, targeted gene (amplicon) sequencing, immune repertoire sequencing, and 16s metagenomics sequencing. We also offer a variety of flow cell types for the MiSeq to better serve a range of sequencing depths and project sizes.
| Flow Cell Type | v2 Nano | v2 Micro | v2 | v3 |
| Clusters PF | Up to 1 million | Up to 4 million | Up to 15 million | Up to 25 million |
| Available Run Configurations | ||||
| PE75, SR150 (150 cycle) | – | – | – | Yes |
| PE150 (300 cycle) | Yes | Yes | Yes | – |
| PE250 (500 cycle) | Yes | – | Yes | – |
| PE300 (600 cycle) | – | – | – | Yes |