Pricing Overview

We have experience with a wide range of techniques and the ability to accommodate a variety of projects. As a guide, we have listed pricing below for some of the more common types of sequencing runs. To obtain a quote or discuss a project type that is not listed here, please contact Kathryn Pellegrini.

Library Prep and Sequencing

Our bulk RNAseq and miRNAseq pricing includes library prep and sequencing as described. Our microbiome (16S) and DNA library prep pricing only includes library prep, as the required sequencing depth will be project specific. Our nucleic acid extraction services are also listed further down the page.

Bulk Sequencing Service Sequencing Configuration <32 Samples 32+ Samples
Conventional mRNAseq 18-20M reads/sample 100SR  $420  $380
High-input, stranded mRNAseq 18-20M reads/sample 100SR  $420  $380
Total RNAseq 25-30M reads/sample 100SR  $450  $400
Paxgene RNAseq (+globin depletion) 25-30M reads/sample 100SR  $480  $430
miRNAseq 8-10M reads/sample 100SR  $360  $340
16S rRNA seq Library prep only  $55  $45
DNA library prep Library prep only  $65  $55

Single Cell Sequencing

Our single cell library prep and sequencing services are priced separately as the number of cells captured will affect the required amount of sequencing. Our standard gene expression assay is run using the 5' 10X Genomics method, and the VDJ and Feature Barcode library preps are available as add-on services to a gene expression capture. 

Single Cell Sequencing Service Pricing Unit Unit Cost
Gene Expression Capture & Library Prep Price/capture (up to 10000 cells)  $1,800
VDJ Library Prep Price/capture  $300
Feature Barcode Library Prep Price/capture  $300
Sequencing of scRNAseq Gene Expression Libraries Price/cell (50000 reads/cell)  $0.28
Sequencing of VDJ or Feature Barcode Libraries Price/cell (5000 reads/cell)  $0.03
ATACseq Nuclei Capture & Library Prep Price/capture (up to 10000 nuclei)  $1,800
Sequencing of scATACseq Libraries Price/nuclei (25000 read pairs/nuclei)  $0.22

Sequencing Only

If you would prefer to prepare your own libraries, we also offer a variety of sequencing options. User-prepared libraries must be run separately in their own lane or flowcell.

NovaSeq Flowcell Run Type Expected Reads/Run Cost/Run
NovaSeq SP 100 cycle kit (100SR, PE50) 0.65-0.8 billion reads  $4,000
NovaSeq S1 100 cycle kit (100SR, PE50) 1.3-1.6 billion reads  $6,150
NovaSeq S2 100 cycle kit (100SR, PE50) 3.3-4.1 billion reads  $12,200
NovaSeq SP 200 cycle kit (PE100, PE75) 0.65-0.8 billion reads  $4,900
NovaSeq S1 200 cycle kit (PE100, PE75) 1.3-1.6 billion reads  $7,700
NovaSeq S2 200 cycle kit (PE100, PE75) 3.3-4.1 billion reads  $16,000
NovaSeq S4 200 cycle kit (PE100, PE75) 8-10 billion reads  $28,000
NovaSeq SP 300 cycle kit (PE150) 0.65-0.8 billion reads  $5,850
NovaSeq S1 300 cycle kit (PE150) 1.3-1.6 billion reads  $9,000
NovaSeq S2 300 cycle kit (PE150) 3.3-4.1 billion reads  $18,750
NovaSeq S4 300 cycle kit (PE150) 8-10 billion reads  $32,500
NovaSeq SP 500 cycle kit (PE250) 0.65-0.8 billion reads  $7,500

 

MiSeq Run Type Expected Reads/Run Cost/Run
MiSeq v3, 600 cycle kit Up to 25 million reads  $1,925
MiSeq v3, 150 cycle kit Up to 25 million reads  $1,260
MiSeq v2 Micro, 300 cycle kit Up to 4 million reads  $765
MiSeq v2 Nano, 500 cycle kit Up to 1 million reads  $650
MiSeq v2 Nano, 300 cycle kit Up to 1 million reads  $570

RNA Extraction

These prices include QC via Qubit and Bioanalyzer or TapeStation. *Please note that extraction projects have a six sample minimum.

RNA Extraction Services Starting Sample Type Cost/Sample
Qiagen RNeasy Mini* Cells/tissue snap frozen or in RLT  $25
Qiagen RNeasy Micro* Cells/tissue snap frozen or in RLT  $28
Qiagen miRNeasy Mini/Micro* Cells/tissue snap frozen or in QIAzol  $25
PreAnalytix Paxgene Blood RNA* Blood in Paxgene RNA tubes  $30

Quality Control

We are happy to provide quality control services for various samples that you have prepared yourself. **Please note that Bioanalyzer projects have a six sample minimum.

QC Service Type Cost/Sample
Bioanalyzer (RNA Nano or RNA Pico)**  $7
Bioanalyzer (High Sensitivity DNA)**  $9
TapeStation (RNA, RNA-HS, D5000-HS, D1000-HS, Genomic DNA)  $6
Qubit (DNA or RNA)  $3

Bioinformatics

We offer a variety of bioinformatics services to support data generated in our genomics core. This can include a basic alignment and annotation of your sequencing data to produce gene count tables and bam files, or further statistical analyses to identify differentially expressed genes and activated pathways. The scope of each project can vary widely, so please contact Kathryn Pellegrini and Greg Tharp for a quote.